PCR is a one man band, the music is produced in my home studio with a PC, various devices and software. Additionally sometimes also a guitar is involved and maybe in the future some vocals are added. I started making music over 10 years ago, first with my acoutic guitar, followed by an electric one. Soon I discovered the use of computer to produce whole songs. I began with a wavteable addon to my Soundblaster and quickly bought a Roland SC-88. Nowadays I use mainly software synths, but a also... Topic: PCR
Polymerase chain reaction (PCR) as a scientific invention, has revolutionized molecular biology and led to real-time PCR and later, real-time reverse transcription PCR (Real-Time RT-PCR). These two techniques enable scientists to conduct PCR detection of amplified gene products and expression analysis of targeted genes. Quantitative polymerase chain reaction (qPCR), also called real-time polymerase chain reaction, is a recent modification to PCR that utilizes fluorescent reporter molecular... Topics: PCR, Real-Time PCR, Real-Time RT-PCR, Gene Expression
The objective of this work is to isolate mARC, XDH1, AAO3, NIA1, SO and ABA3/LOS5 genes in three legume species submitted to salt stress with molybdenum and nitrogen, the growth conditions (T1: control, T2: both molybdenum and nitrogen added, T3: sodium chloride were added, T4: molybdenum, nitrogen and sodium chloride were added), we found these gene sequences in Phaseolus vulgaris and Cicer arietinum in NCBI but not forLens culinaris, so we tried to isolate them using bean and chickpea primers... Topics: molybdoenzymes, legumes, salt stress, reverse transcriptase PCR.
byIvan Milenkovic, Nenad Keca, Dragan Karadzic, Justyna A. Nowakowska, Malgorzata Borys, Katarzyna Sikora, Tomasz Oszako
According to many surveys of pathogenic organisms in forest soils, the presence of the Phytophthora genus is very common in both dominant and mixed stands of European beech. In Serbia, Phytophthora species were isolated from rhizosphere soil in declining, as well as apparently healthy stands. After detailed morphological and molecular identification, several Phytophthora species were confirmed. The most common pathogen of fine roots in Serbian European beech stands was Phytophthora plurivora... Topics: Oomycetes, Fagus sylvatica L., PCR, ITS sequencing
Abstract — The frequent occurrence of epidemics even after the launching of the Integrated Diseases Surveillance Programme (IDSP) was an indication toward inadequacy of the control system. These epidemics/outbreaks may be identified if disease status analysis is done properly. The aim of the this study was to find out status of some of major diseases included in the IDSP in a tertiary level hospital of western Rajasthan. It was a record-based analysis carried out in hospitals attached to SMS... Topics: Communicable Diseases, Survillance, IDSP, PCR, PDR, CFR
Helicobacter species is now-a-days found commonly in the liver, stomach and intestine of human beings and animals. It has now been detected in pancreas of the human beings also. It is the cause of cancer as it grows into the tissue and is circulated around the body through blood stream and spreads in many organs. Where-ever it finds the niche it grows. In our study, Helicobacter species were identified in tissue surrounding liver cancer and pancreatic tumours from patients undergoing... Topics: Helicobacter, Liver, Pancreas, Human, PCR, Sequencing
The aim of this study is to deploy best conditions of previously published protocols on Agrobacterium-mediated transformation of tomato, to deliver PtdIns-PLC2 gene, which render plants tolerant to drought, to Money Maker, Super Strain B and Rio Grande tomato cultivars. The obtained results indicated that the efficiency of Agrobacterium-mediated gene transfer in tomato tissues is influenced by many factors; the most important are cultivars, explants type and optical density. The highest... Topics: Drought, Agrobacterium tumefaciensis, PtdIns-PLC2 Gene, PCR, RT PCR and GUS Assay
Book collection of protocols in molecular biology and medicine. These books are very useful for research laboratories which are standardizing technics and also to solve troubleshooting. Medicine protocols books are very useful for medicine research, these books include cancer, virus, diabetes, etc, research. Topics: Protocols, medicine, molecular biology, blotting, PCR, RT-PCR, chromatography, virus, cancer
Polymerase chain reaction (PCR) as a scientific invention, has revolutionized molecular biology and led to real-time PCR and later, real-time reverse transcription PCR (Real-Time RT-PCR). These two techniques enable scientists to conduct PCR detection of amplified gene products and expression analysis of targeted genes. Quantitative polymerase chain reaction (qPCR), also called real-time polymerase chain reaction, is a recent modification to PCR that utilizes fluorescent reporter molecular... Topics: International Journal of Genetics and Genomics:PCR, Real-Time PCR, Real-Time RT-PCR, Gene Expression
A collection of protocols in molecular biology books, these books are very usefull for research laboratories which are developing new technics, also for troubleshooting and FAQs in molecular biology technics such as western, northern and southern blotting, ELISA, DNA cloning, etc. This collections includes medicine protocols for medicine research. Topics: Protocols, medicine, molecular biology, blotting, PCR, RT-PCR, chromatography, virus, cancer
DNA manipulation 2 - Enzymes to modify, label, amplify or quantify nucleic acids. DNA polymerases, labels, polymerase chain reaction (PCR), quantitative PCR, Real Time-PCR Topics: DNA manipulation, Enzymes to modify, label, amplify or quantify nucleic acids. DNA polymerases,...
RAPD was used to determine the molecular genetic diversity of four chicken species viz. White Plymouth Rock – Rhode Island, commercial broiler and Aseel. Using eight oligo primers. a total 68 amplicons (average 8.5per primer) were obtained of which 51 polymorphic. Total 68 banding pattern were observed with an average of 7.6 per primer followed by 0.82% average discriminatory power, and 0.38 average PIC value. Based on UPGMA, Jaccard`s coefficient similarity range from 17 % (RIR2 and CB1) to... Topics: Molecular Marker, RAPD, PCR, Genetic Diversity, Poultry, Chicken
byBadria F. Essammak, Mohammed J. Ashour, Fadel A. Sharif
Hearing loss is a common, pan-ethnic and highly heterogeneous sensory disorder with an incidence of around 1 in 1000 infants. Genetic causes are thought to be responsible for more than 60% of the cases with the majority of non-syndromic hearing impairment being inherited in an autosomal recessive pattern. The gene that is most frequently mutated in autosomal recessive non-syndromic hearing loss (ARNSHL) is gap junction protein beta-2 (GJB2) which codes for connexin 26 (Cx26). Cx26 plays a key... Topics: ARNSHL, Cx26, GJB2, Mutation, PCR-RFLP, Gaza Strip
Blackgram (Vigna mungo (L.) hepper), is one of the important pulse crops in the world especially in India, it is cultivated both in Kharif and Rabi seasons under semi irrigated and dry land conditions, where the drought stress is a major problem. Plants adapt alternate pathways for their existence during the periods of stress, especially at flowering and pod filling stages. It is evident that resistance or sensitivity to the stress depends on species, genotypes and even developmental stages and... Topics: Lithium Chloride, RNA, Drought Stress and DEPC, RT-PCR
The follow up to his AMAZING EP, 'the earth is gray" finds Conceptual Reality exploring some slightly new territories, with some more ambient and electronically-focused tracks. His signature mastery of melody remains in tact throughout. Topics: Conceptual Reality, PCR, Post-Rock, instrumental, the earth is gray
DNA manipulation 2 - Enzymes to modify, label, amplify or quantify nucleic acids. DNA polymerases, labels, polymerase chain reaction (PCR), quantitative PCR, Real Time-PCR Topics: DNA manipulation 2 - Enzymes to modify, label, amplify or quantify nucleic acids. DNA polymerases,...
byXinde Li, Xinhan Huang, Min Wang, Jiansheng Xu, Hongbo Zhang
In this paper, considering the information fusion of multi-sensors on mobile robot, a new tool (the fusion machine of DSmT (Dezert-Smarandache Theory) coupling with PCR5 (Proportional Conflict Redistribution Rule)) is applied to autonomous mobile robot’s map reconstruction from 16 sonar rangefinders. Topics: DSmT, PCR, Information Fusion, Mobile Robot, Map Reconstruction
an experimental-folk-freak-out. Songs of mortality, religion, and the meaning of life. Despite the acoustic guitars, it gives a distinct feeling that the end is coming soon. Topics: elis, PCR, folk, post-rock, ambient, experimental, acoustic, living
The follow up to ZH's previous release on PCR, "Phantasm". Even more abstract than his first outing, "Apparition" takes you places you never knew existed. Topics: ZH, apparition, PCR, post-rock, ambient, instrumental, electronic
Helicobacter pullorum is the bacterium found in the poultry and is an infective intestinal pathogen of man. 40 BALB/cA mice were used for the experiment to investigate whether the mice get infected with H. pullorum or not. We analysed stomach, intestine, caecum, liver and faeces of the mice after infection at every two weeks interval and found to be culture and PCR positive. The controls showed negative results. Immunoblotting of serum samples from infected mice showed positive bands of... Topics: H. pullorum, Mice, Liver, Stomach, Intestine, Faeces, PCR
In this paper we propose five versions of a Proportional Conflict Redistribution rule (PCR) for information fusion together with several examples. From PCR1 to PCR2, PCR3, PCR4, PCR5 one increases the complexity of the rules and also the exactitude of the redistribution of conflicting masses. PCR1 restricted from the hyper-power set to the power set and without degenerate cases gives the same result as the Weighted Average Operator (WAO) proposed recently by Jøsang, Daniel and Vannoorenberghe... Topics: PCR rules, Dezert-Smarandache classic and hybrid rules, DSmT
DNA markers are currently the most precise tool for forest tree species identification and can be used for comparative analyses of plant material. Molecular diagnosis of evidence and reference material is based on comparing the structure of DNA markers duplicated in the PCR reaction and estimation of the DNA profiles obtained in studied wood samples. For this purpose, the microsatellite DNA markers are the most suitable tool because of their high polymorphism and accurate detection of... Topics: stolen wood, wood molecular identification, PCR-RFLP markers, SSR, STS
A blend of ambient music and post-rock goodness, this album will make you drift off into your own mind and then awaken you suddenly with urgency. Topics: Contest, waking self, PCR, post rock, ambient, instrumental
In India, silk textile is economically important industry and Karnataka alone accounts for 63% of the country’s silk production. The silk industry is affected by many fungal and viral major diseases and thus there is decrease in the silk production. In this study the early detection of the viral disease caused by Kenchu virus to silkworm (BmKV) was studied by molecular and immunological methods. The Kenchu virus isolate was collected from Vijayapura, Karnataka, India and ultrapurified using... Topics: Bombyxmori, Kenchu Virus, PCR, Dot-ELISA, Plate ELISA
Electronic beats sprinkled with ambient and post-rock-esque music. An interesting take on the genre, your collection wouldn't be complete without this oddball gem of a release. Topics: electronic, ambient, post-rock, instrumental, talbot arms, pcr
Extracellular peroxidases and laccases catalyze the breakdown of organic pollutants via free radical–mediated reactions. In this study, the ligninolytic activities of selected strains of Pleurotus ostreatus were determined in a nitrogensufficient liquid medium. In order to determine the effect of temperature in ligninolytic activities, the white rot fungi were incubated at different cultivation temperatures (15, 20, 25 and 30οC). The activities of manganese peroxidase and laccase were... Topics: Biodegradation, Lindane, Pleurotus Ostreatus, Laccase, PCR, Central Composite Design
Dengue is a fast emerging pandemic-prone viral disease in many parts of the world. The objective of the study was to report the seroprevalence of dengue virus infection among clinically suspected pediatric patients reporting to children’s hospital from August 2011 to October 2011 in Chennai, Tamilnadu, India. This retrospective study was analyzed from 100 dengue patients reporting to hospital. Of these, 94 serum samples were subjected to IgM ELISA and confirmed by RT-PCR and also to find out... Topics: Seroprevalence, Dengue Fever, IgM ELISA, RT-PCR, Childrens
Life Cycle Assessment (LCA) studies are designed to be used by designers, manufacturers, engineers and companies, howeverthere are very few that would be readily understood by consumers. This article explores a new perspective of sustainability assessment methods from the retailers’ perspective on LCA information and the consumers’ viewpoint in order to provide consumers with easy-to-understand and meaningful sustainability information, influence shopping behavior and therefore stimulate... Topics: Life Cycle Assessment (LCA), Near Field Communication (NFC), Sustainability, Product Category Rules...
El pasado viernes 25 de septiembre, fue desalojada brutalmente la fÃ¡brica de Kraft, que se encontraba ocupada por los mÃ¡s de 160 trabajadores despedidos arbitrariamente. Casi inmediatamente, a las 19:00hs, trabajadores y estudiantes marcharon desde el centro hacia 120 y 32, donde se encuentra la bajada de la Autopista Buenos Aires-La Plata La FULP, SUTEBA La Plata, el MAS, el PO, el PTS y el PCR, cortaron durante mÃ¡s de una hora la bajada de la autopista en solidaridad con los... Topic: Kraft Terrabusi Trabajadores RepresiÃ³n La Plata corte de autopista protesta FULP MAS PTS PO PCR
Foot rot caused by Phytophthora capsici is a major soil borne disease in black pepper, known as the king of spices. Piper colubrinum a distant relative of black pepper is known to be resistant to foot rot disease. The induction of PR proteins and defense response genes during pathogen stress is known to be an important strategy for plants to control the spread of infection during the initial stages of attack by the pathogen. Here we investigated the role of PR proteins viz osmotin,... Topics: Osmotin, β-1, 3-Glucanase, Defensin, Thaumatin-Like Protein, Real Time PCR, P. Colubrinum, P.Capsic
byFrancois Lefort, Julien Crovadore, Romain Gilodi, Gautier Calmin, Tomasz Oszako, Justyna A. Nowakowska
A model for tree pathogen diagnosis – Prunus domestica L. has been studied against pathogenic bacteria. An orchard of 110 trees of P. domestica showed dying back symptoms in May 2009 and nineteen of these trees were eradicated and burnt for prophylaxis. No symptoms correlated with those caused by pathogens previously observed in stone fruit die back in Europe or elsewhere (Pseudomonas syringae pv syringae van Hall, Pseudomonas syringae pv morsprunorum Lazarowtz, Phytophthora sp., Diaporthe... Topics: PCR and sequencing diagnostic, Prunus domestica, Pseudomonas, rootstock, stone fruit die back
This is the book by Kary Mullis, Dancing Naked in the Mind Field, made with Adobe Acrobat from the EPUB a few hours ago into this PDF. Please enjoy! Published: October 1997 Topics: DNA, PCR, Molecular Biology, Kary Mullis, Dancing Naked in the Mindfield
Foxtail millet (Setaria italica L.) is an important food and fodder grain crop in arid and semi-arid regions of Asia and Africa. Being an elite drought-tolerant crop, foxtail millet is thought to be an excellent experimental model in studying abiotic stress tolerance system. In recent years, semi-quantitative RT-PCR has emerged as a versatile technique in transcriptomics, as it can generate rapid measurement of mRNA levels in minimal tissue samples. The present study deals with... Topics: Differential Expression Profiling, Semi-Quantitative RT-PCR, Setaria italica, Abiotic Stress
http://www.royotyreindustrial.com/ RoyoTyre Industrial Corp Ltd is committed to provide quality tires and professional service to worldwide customers since founded in 2003 Topics: Agricultural tyres, TBR tyres china, PCR Tyre, China agricultural tyres, agricultural tyres for sale
byDaniel Azubuike Awomukwu, Bio Louis Nyananyo, Paula Spies, Bulelani Londoloza Sizani
An extraction, purification, PCR amplification and sequencing of DNA from five species of Phyllanthus in Nigeria namely P. amarus Schum and Thonn, P. urinaria Linn., P. odontadenius Mull-Arg., P. niruroides Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were carried out using nuclear ribosomal Internal Transcribed Spacer (ITS 4-5) genetic marker to identify unknown Phyllanthus species. The nuclear region revealed that the Phyllanthus species were able to... Topics: Phyllanthus species, DNA Extraction, PCR Amplification, Nucleotide Sequences, Gen Bank, Bold...